Title:
【発明の名称】肝臓の持続的な再生およびC型肝炎の処置のための組換えベクター
Document Type and Number:
Japanese Patent JPH10510815
Kind Code:
A
Abstract:
A method of inhibiting hepatitis C virus RNA replication or expression is provided. The method consists of introducing two or more ribozymes specific for hepatitis C virus RNA into a cell infected with hepatitis C virus. The ribozymes specific for hepatitis C virus RNA can specifically cleave hepatitis C RNA in a HCV 5' non-coding sequence, the capsid sequence, the NS-5 sequence or any other conserved region of the hepatitis C RNA. The ribozymes can also be selected so as to be specific for opposite strands of the virus genome. A method of inhibiting hepatitis C virus RNA replication or expression is also provided which consists of introducing into a cell infected with hepatitis C virus at least one ribozyme specific for hepatitis C virus which is selected from the group consisting of GGGAGGTCTCGTAGA [SEQ ID NO: 1], GCACCATGAGCACGA [SEQ ID NO: 2], CCCACAGGACGTCAA [SEQ ID NO: 3], CAACCGTCGCCCACA [SEQ ID NO: 4], TAAACCTCAAAGAAA [SEQ ID NO: 5] GTAAGGTCATCGATA [SEQ ID NO: 6]. Compositions consisting of two or more ribozymes specific for hepatitis C virus RNA is also provided.
Inventors:
Kay, Mark A.
Lever, Andre
Lever, Andre
Application Number:
JP51927496A
Publication Date:
October 20, 1998
Filing Date:
December 14, 1995
Export Citation:
Assignee:
University of Washington
International Classes:
C12N15/09; A61K31/70; A61K35/76; A61K38/46; A61K38/48; A61K48/00; A61P1/16; A61P31/12; C12N7/00; C12N9/72; C12N15/113; (IPC1-7): A61K48/00; A61K35/76; A61K38/46; C12N7/00; C12N15/09
Attorney, Agent or Firm:
Shusaku Yamamoto