Title:
【発明の名称】架橋性オリゴヌクレオチド
Document Type and Number:
Japanese Patent JPH11509528
Kind Code:
A
Abstract:
Oligonucleotides (ODNs) include a sequence that is complementary to a target sequence in single stranded RNA, or single or double stranded DNA, and an alkylating function which after hybridization alkylates the target sequence. ODNs adapted for alkylating single stranded RNA, such as messenger RNA, are complementary to the target sequence in the Watson Crick sense. ODNs adapted for alkylating double stranded DNA have at least two alkylating functions and are complementary to the target sequence in the Hoogsteen or reverse Hoogsteen sense. With these ODNs both strands of the target sequence are alkylated. A third class of ODNs have at least approximately 26 nucleotide units in a continous sequence which are complementary to the target sequence of double stranded DNA, and the alkylating function is covalently attached to a nucleotide unit in the continuous sequence. Alkylation or cross-linking with this class of ODNs occurs in the presence of a recombinase enzyme.
Inventors:
Mayer, Rich B. Junior
Gunper, howard bee
Cut Yabin, Igor Buoy
Goal, alexander a
Petrie, Charles Earl
Tabourne, John Sea
Hurst, gerald di
Gunper, howard bee
Cut Yabin, Igor Buoy
Goal, alexander a
Petrie, Charles Earl
Tabourne, John Sea
Hurst, gerald di
Application Number:
JP50184997A
Publication Date:
August 24, 1999
Filing Date:
June 07, 1996
Export Citation:
Assignee:
Epoch Pharmaceuticals Incorporated
International Classes:
A61K31/70; A61K48/00; A61P31/00; A61P35/00; C07H21/00; C12N15/10; C12N15/113; C12Q1/68; A61K38/00; (IPC1-7): C07H21/00; A61K48/00; C12Q1/68
Attorney, Agent or Firm:
Aoyama Ryo (2 outside people)