Document Type and Number:
Japanese Patent JPS4849989
Kind Code:
A
Abstract:
1385274 Zeaxanthin NESTLE SA 26 Oct 1972 [27 Oct 1971 24 May 1972] 49475/72 Heading C2V A microorganism of the genus Flavobacter is cultured until zeaxanthin production starts then cultivation proceeds at 22-25‹ C., with oxygenation, in an aqueous nutrient containing at least one carbohydrate (15-35 mg/l as assimilable carbon) and at least one source of assimilable amino nitrogen containing free amino acids, the amount of carbon source being maintained substantially constant by additions of nutrient containing said source during the cultivation. The pH of the medium is preferably maintained at 6À5-8À0. The carbohydrate may be glucose, lactose or sucrose and the nitrogen source may be NH 3 , corn steep liquor, yeast extract, a protein hydrolysate or a hydrolysate of a Flavobacter biomass. After fermentation the cells may be separated and treated with a polar organic solvent to extract the zeaxanthin. The initial cultivation may be in a medium containing excess of the carbon source which is allowed to fall to within the specified limits for the main cultivation at constant carbon source concentration. The microorganism may be a mutant of a genus Flavobacter bacterium.
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Application Number:
JP10780372A
Publication Date:
July 14, 1973
Filing Date:
October 27, 1972
Export Citation:
International Classes:
C12P17/18; C12P23/00; C12R1/20; (IPC1-7): C12D5/00