Document Type and Number:
Japanese Patent JPS5016677
Kind Code:
B1
Abstract:
1,189,544. Determining alcohols. SMITH KLINE & FRENCH LABORATORIES. 15 July, 1968 [26 July, 1967], No.33716/68. Heading B1X. An anhydrous reagent for determining the ethanol content of body fluids comprises alcohol dehydrogenase, N.A.D., a buffer, an acid-neutralizing agent, and, as trapping agent for the acetaldehyde formed, a C 2 to C 5 aminoxyalkanoic acid or a C 1 to C 4 amino-oxyalkane sulfonic acid. The reaction used is:- C 2 H 5 OH+NAD#KH 3 CHO+NADH+ H+ The NADH is determined by measuring its absorption at 340mÁ. The reaction may also be used to determine the dehydrogenase by replacing the dehydrogenase in the reagent by alcohol; or to determine the activity of trypsin in releasing ethanol from benzoyl-arginine ethyl ester; to determine lactic acid or betahydroxybutyric acid by using lactate dehydrogenase or beta-hydroxybutyric acid dehydrogenase in the reagent instead of the alcohol dehydrogenase, and generally to determine the activity of proteases or esterases which liberate ethanol, propanol or butanol. The reagent may be packed in single assay amounts in genatin capsules. A stabilized form of alcohol dehydrogenase may be obtained by freeze-drying a solution containing gum acacia, NAD, cysteine hydrochloride, glycine, albumins, tris-versene buffer and the dehydrogenase.
Application Number:
JP4742968A
Publication Date:
June 14, 1975
Filing Date:
July 06, 1968
Export Citation:
International Classes:
C12Q1/32; G01N33/497; G01N33/02; G01N33/98; (IPC1-7): G01N31/14