Title:
【発明の名称】ヒト成長ホルモンの製造方法
Document Type and Number:
Japanese Patent JPS62501609
Kind Code:
A
Abstract:
A desired protein having the formula: A-B-C-P wherein a) A is Lys, Arg, and B and C are arbitrary amino acids, or b) A is a arbitrary amino acid different from Pro, Lys and Arg, and B and/or C is Pro, is produced from a biosynthetically formed amino acid extended protein having the formula: X-A-B-C-P wherein A, B, C and P are as defined above, and X is an amino acid sequence with an even number of amino acids, of which the first one, seen from the N-terminal end, is different from Lys and Arg, all other uneven amino acids are different from Pro, Lys and Arg, and all even amino acids are different from Pro, by reaction with the enzyme dipeptidyl aminopeptidase (DAP I). The desired protein is obtained in a pure state. Thus, e.g. hGH without content of Met-hGH may be produced by the process.
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Inventors:
Anderson, Henrik, Dalboge
Jedeon, Pedersen
Christensen, sole killed
Hansen, The Orli, Winnie
Jiessen, Tolben, Allern
Jedeon, Pedersen
Christensen, sole killed
Hansen, The Orli, Winnie
Jiessen, Tolben, Allern
Application Number:
JP50120686A
Publication Date:
July 02, 1987
Filing Date:
February 06, 1986
Export Citation:
Assignee:
Novo-Nordisk A/S
International Classes:
C07K14/545; C07K14/61; C12N9/22; C12N15/00; C12N9/36; C12N15/09; C12N15/62; C12P21/00; C12P21/04; C12P21/06; (IPC1-7): C12P21/06; C12N15/00
Attorney, Agent or Firm:
Takehiko Suzue (2 outside)