Title:
NICOTIANAMINE SYNTHASE AND GENE ENCODING THE SAME
Document Type and Number:
WIPO Patent Application WO/1999/057249
Kind Code:
A1
Abstract:
A nicotianamine synthase is isolated and purified. Then the gene of this enzyme is cloned and the base sequence and amino acid sequence thereof are determined. This gene is employed in constructing plants, in particular, grass plants highly tolerant to iron deficiency. A nicotianamine synthase involved in the mugineic acid biosynthesis pathway; the amino acid sequence thereof; a gene encoding the same; a vector containing this gene; cells transformed by the vector; a process for producing nicotianamine by using the same; plants transformed by the gene encoding the nicotianamine synthase; and an antibody against the nicotianamine synthase.
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Inventors:
MORI SATOSHI (JP)
HIGUCHI KYOKO (JP)
SUZUKI KAZUYA (JP)
NISHIZAWA NAOKO (JP)
NAKANISHI HIROMI (JP)
HIGUCHI KYOKO (JP)
SUZUKI KAZUYA (JP)
NISHIZAWA NAOKO (JP)
NAKANISHI HIROMI (JP)
Application Number:
PCT/JP1999/002305
Publication Date:
November 11, 1999
Filing Date:
April 30, 1999
Export Citation:
Assignee:
JAPAN SCIENCE & TECH CORP (JP)
MORI SATOSHI (JP)
HIGUCHI KYOKO (JP)
SUZUKI KAZUYA (JP)
NISHIZAWA NAOKO (JP)
NAKANISHI HIROMI (JP)
MORI SATOSHI (JP)
HIGUCHI KYOKO (JP)
SUZUKI KAZUYA (JP)
NISHIZAWA NAOKO (JP)
NAKANISHI HIROMI (JP)
International Classes:
C12N9/10; C12N15/82; (IPC1-7): C12N9/00; C12N15/52; C12P13/04; C07K16/40
Other References:
HIGUCHI K, ET AL.: "PURIFICATION AND CHARACTERIZATION OF NICOTIANAMINE SYNTHASE FROM FE-DEFICIENT BARLEY ROOTS", PLANT AND SOIL., KLUWER ACADEMIC PUBLISHERS, DORDRECHT., NL, vol. 165, 1 January 1994 (1994-01-01), NL, pages 173 - 179, XP002919794, DOI: 10.1007/BF00008059
HIGUCHI K, ET AL.: "CLONING OF NICOTIANAMINE SYNTHASE GENES, NOVEL GENES INVOLVED IN THE BIOSYNTHESIS OF PHYTOSIDEROPHORES", PLANT PHYSIOLOGY, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, USA, vol. 119, 1 January 1999 (1999-01-01), Rockville, Md, USA, pages 471 - 479, XP002919795, ISSN: 0032-0889, DOI: 10.1104/pp.119.2.471
DATABASE MPSRCH GENBANK 1 January 1900 (1900-01-01), MORI S, HIGUCHI K: "Hordeum Vulgare Hvnas7 mRNA for Nicotianamine Synthase 7, Complete Cds", XP002947405, Database accession no. AB019525
DATABASE MPSRCH GENBANK 1 January 1900 (1900-01-01), MORI S, ET AL: "Oryza Sativa Osnasl mRNA for Nicotianamine Synthase 1, Complete Cds", XP002947406, Database accession no. AB021746
DATABASE MPSRCH GENBANK 1 January 1900 (1900-01-01), SUZUKI K, MORI S: "Arabidopsis Thaliana Gene for Nicotianamine Synthase, Complete Cds", XP002947407, Database accession no. AB021934
See also references of EP 1077255A4
HIGUCHI K, ET AL.: "CLONING OF NICOTIANAMINE SYNTHASE GENES, NOVEL GENES INVOLVED IN THE BIOSYNTHESIS OF PHYTOSIDEROPHORES", PLANT PHYSIOLOGY, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, USA, vol. 119, 1 January 1999 (1999-01-01), Rockville, Md, USA, pages 471 - 479, XP002919795, ISSN: 0032-0889, DOI: 10.1104/pp.119.2.471
DATABASE MPSRCH GENBANK 1 January 1900 (1900-01-01), MORI S, HIGUCHI K: "Hordeum Vulgare Hvnas7 mRNA for Nicotianamine Synthase 7, Complete Cds", XP002947405, Database accession no. AB019525
DATABASE MPSRCH GENBANK 1 January 1900 (1900-01-01), MORI S, ET AL: "Oryza Sativa Osnasl mRNA for Nicotianamine Synthase 1, Complete Cds", XP002947406, Database accession no. AB021746
DATABASE MPSRCH GENBANK 1 January 1900 (1900-01-01), SUZUKI K, MORI S: "Arabidopsis Thaliana Gene for Nicotianamine Synthase, Complete Cds", XP002947407, Database accession no. AB021934
See also references of EP 1077255A4
Attorney, Agent or Firm:
Saeki, Norio (9th floor 15-2, Nihonbashi 3-chome Chuo-ku Tokyo, JP)
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