Title:
FUSION PROTEIN OF ONCONASE AND TOXIN TRANSMEMBRANE DOMAIN AND PREPARATION METHOD AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2009/155842
Kind Code:
A1
Abstract:
The invention provides a fusion protein of onconase (Onc) and bacteria toxin transmembrane domain, a DNA sequence encoding the fusion protein, a vector comprising the DNA sequence, a host cell containing the vector and the method for preparing the fusion protein by genetic engineering. The fusion protein of the invention not only retains the activity of the Onc itself but also has more strong ability for killing the tumor cell, thereby can be used for board-spectrum anti-tumor therapy.
Inventors:
WANG QINGCHENG (CN)
SHEN RULING (CN)
SUN RUILIN (CN)
FEI JIAN (CN)
LI JUN (CN)
SHEN RULING (CN)
SUN RUILIN (CN)
FEI JIAN (CN)
LI JUN (CN)
Application Number:
PCT/CN2009/072368
Publication Date:
December 30, 2009
Filing Date:
June 22, 2009
Export Citation:
Assignee:
SHANGHAI BIOMODEL ORGANISM SCI (CN)
SHANGHAI RES CT FOR MODEL ORGA (CN)
WANG QINGCHENG (CN)
SHEN RULING (CN)
SUN RUILIN (CN)
FEI JIAN (CN)
LI JUN (CN)
SHANGHAI RES CT FOR MODEL ORGA (CN)
WANG QINGCHENG (CN)
SHEN RULING (CN)
SUN RUILIN (CN)
FEI JIAN (CN)
LI JUN (CN)
International Classes:
C07K19/00; A61K38/16; A61P35/00; C07K14/195; C12N9/22; C12N15/62; C12N15/63; C12P21/02
Other References:
PRIOR, T. I. ET AL.: "Translocation mediated by domain II of Pseudomonas exotoxin A: transport of bamase into the cytosol.", BIOCHEMISTRY, vol. 31, no. 14, 14 April 1992 (1992-04-14), pages 3555 - 3559
ERICHKSON, H. A. ET AL.: "Cytotoxicity of human RNase-based immunotoxins requires cytosolic access and resistance to ribonuclease inhibition.", PROTEIN ENGINEERING, DESIGN & SELECTION., vol. 19, no. 1, 21 October 2005 (2005-10-21), pages 37 - 45
ARDELT, W. ET AL.: "Amino acid sequence of an anti-tumor protein from Rana pipiens oocytes and early embryos.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 266, no. 1, 5 January 1991 (1991-01-05), pages 245 - 251
BENNETT, M. J. ET AL.: "Refined structure of monomeric diphtheria toxin at 2.3 A resolution.", PROTEIN SCIENCE., vol. 3, no. 9, September 1994 (1994-09-01), pages 1464 - 1475
TSUNEOKA, M. ET AL.: "Evidence for involvement offurin in cleavage and activation of diphtheria toxin.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 35, 15 December 1993 (1993-12-15), pages 26461 - 26465
ERICHKSON, H. A. ET AL.: "Cytotoxicity of human RNase-based immunotoxins requires cytosolic access and resistance to ribonuclease inhibition.", PROTEIN ENGINEERING, DESIGN & SELECTION., vol. 19, no. 1, 21 October 2005 (2005-10-21), pages 37 - 45
ARDELT, W. ET AL.: "Amino acid sequence of an anti-tumor protein from Rana pipiens oocytes and early embryos.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 266, no. 1, 5 January 1991 (1991-01-05), pages 245 - 251
BENNETT, M. J. ET AL.: "Refined structure of monomeric diphtheria toxin at 2.3 A resolution.", PROTEIN SCIENCE., vol. 3, no. 9, September 1994 (1994-09-01), pages 1464 - 1475
TSUNEOKA, M. ET AL.: "Evidence for involvement offurin in cleavage and activation of diphtheria toxin.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 35, 15 December 1993 (1993-12-15), pages 26461 - 26465
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (Xuhui, Shanghai 3, CN)
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