To solve a problem in that soyasapogenol B is bio-synthesized by the ring closure of 2,3-oxidosqualene produced from β-amyrin which is a bio-synthetic precursor of the soyasapogenol B, through a mevalonic acid pathway and further through two stage hydroxylation reactions, however a gene of 22 position hydroxylase associating with the reaction is not yet elucidated.
It is found out that the 22 position of an oleanane type triterpene is hydroxylated by specifying the gene of the hydroxylase of 22 position of the oleanane type triterpene and co-expressing by combining with a specific gene. Further, it is found out that by co-expressing the gene of the 22 position hydroxylase with the gene of 24 position hydroxylase, the soyasapogenol B can be produced in a large amount and in a good efficiency.
SHIBUYA MASAAKI
WAKITA ERIKO
Kenjiro Yamaguchi