Title:
付加物保護分析
Document Type and Number:
Japanese Patent JP3577085
Kind Code:
B2
Abstract:
The present invention features an adduct protection assay involving the use of a labelled binding partner and a signal altering ligand. The signal altering ligand can preferentially alter the ability of label which is not part of a binding partner:analyte complex to produce a detectable signal, compared to its ability to alter signal produced from label which is part of a binding partner:analyte complex. The presence or amount of analyte can be determined by detecting the signal produced from unaltered label. The adduct protection assay is very versatile. For example, alteration of signal can be carried out under a wide range of conditions (e.g., pH, temperature, and ionic strength), and both label alteration and signal triggering can be carried out at essentially constant temperature to achieve a high degree of sensitivity.
Inventors:
Becker, Michael
Nelson, Norman See
Nelson, Norman See
Application Number:
JP50074597A
Publication Date:
October 13, 2004
Filing Date:
May 23, 1996
Export Citation:
Assignee:
Gen-Probe Incorporated
International Classes:
C07D219/04; C07H21/00; C12Q1/68; G01N33/542; G01N33/543; G01N33/58; (IPC1-7): G01N33/58; C07D219/04; C12Q1/68
Domestic Patent References:
| JP2503268A |
Foreign References:
| WO1995003427A1 |
Attorney, Agent or Firm:
Kazuo Shamoto
Shosuke Imai
Tadashi Masui
Tadahiko Kurita
Yasushi Kobayashi
Reiko Tanaka
Shosuke Imai
Tadashi Masui
Tadahiko Kurita
Yasushi Kobayashi
Reiko Tanaka