Title:
ALKALI PULLULANASE AND ITS PRODUCTION
Document Type and Number:
Japanese Patent JP2814178
Kind Code:
B2
Abstract:
PURPOSE: To obtain a new alkali pullulanase useful as an ingredient for cleansing agents for dishes and detergents for clothing.
CONSTITUTION: An alkali pullulanase has properties capable of producing maltotriose by decomposition of α-1,6-glucoside bond, hydrolyzing α-1,6-glucoside bond of starch, aminopectin, etc., and specifically hydrolyzing sugar having branched structure with degree of polymerization not lower than that of maltotriose, reacting at reactional pH of 6.5-10.5 (optimum pH of 8.5-9.5) and 35-60°C (optimum temperature of about 50°C) and has 115000±5000 molecular weight (measured by SDS electrophoresis). The alkali pullulanase is obtained by culturing a transformed microorganism consisting of a pair of bases of about 6.3 Kb coding alkali pullulanase and transformed with a recombinant vector containing a DNA fragment having a restriction enzyme map of the Figure.
Inventors:
Katsutoshi Ara
Igarashi Kazuaki
Katsuhisa Saeki
Kawai Shuji
Susumu Ito
Igarashi Kazuaki
Katsuhisa Saeki
Kawai Shuji
Susumu Ito
Application Number:
JP3839093A
Publication Date:
October 22, 1998
Filing Date:
February 26, 1993
Export Citation:
Assignee:
Kao Corporation
International Classes:
C12N1/21; C12N9/44; C12N15/09; C12N15/56; C12R1/07; C12R1/19; (IPC1-7): C12N9/44; C12N15/09
Other References:
Ｂｉｏｓｃｉ．Ｂｉｏｔｅｃｈｎｏｌ．Ｂｉｏｃｈｅｍ．５６（１）１９９２ ｐ．６２−６５
Attorney, Agent or Firm:
Miyuki Ariga (3 outside)