アルカリホスファターゼ組成物並びに脱リン酸化核酸及び標識化核酸の製造方法

Title:

アルカリホスファターゼ組成物並びに脱リン酸化核酸及び標識化核酸の製造方法

Document Type and Number:

Japanese Patent JP7402429

Kind Code:

B2

Abstract:

An object of the present invention is to provide a composition containing an alkaline phosphatase and having a high quality, a method for producing a dephosphorylated nucleic acid by using the composition and a method for producing a labeled nucleic acid by using the composition, and in order to achieve the object, the present invention provides a composition containing:an alkaline phosphatase; anda peptide fragment group (A) composed of two or more peptide fragments, wherein each of the two or more peptide fragments consists of 5 to 50 consecutive amino acid residues selected from positions 501 to 578 of the amino acid sequence set forth in SEQ ID NO: 5,wherein a content ratio of the peptide fragment group (A) to the alkaline phosphatase satisfies the following formula (A): XA/Y×100≤4.4000wherein X_A represents a peak area value of the peptide fragment group (A) calculated by an automatic integration method from an extracted ion chromatogram obtained by an LC-MS/MS analysis of the composition, and Y represents a peak area value of the alkaline phosphatase calculated by an automatic integration method from a chromatogram obtained by an LC-UV analysis of the composition.

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Inventors:

Yoji Ueda
Yuki Takii
Masateru Ito
Mai Yagi

Application Number:

JP2019554584A

Publication Date:

December 21, 2023

Filing Date:

September 25, 2019

Export Citation:

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Assignee:

Toray Industries, Inc.

International Classes:

C12N9/16; C12P19/34; C12Q1/6806

Foreign References:

US20080098491
US20070148140

Other References:

POZIDIS C and BOURIOTIS V,Preparation of high purity alkaline phospatase from calf intestine using dye-ligand chromatography,Bioseparation,1995年,vol.5,no.2,p.89-93

Attorney, Agent or Firm:

Hiroyuki Nagai
Yukitaka Nakamura
Satoru Asakura
Mari Asano
Hiroshi Sorimachi
Keiyasu Suzuki

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