Title:
ALPHA(1,2)FUCOSYLTRANSFERASES SUITABLE FOR USE IN PRODUCTION OF FUCOSYLATED OLIGOSACCHARIDES
Document Type and Number:
Japanese Patent JP2018093883
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide compositions and methods for engineering E. coli or other host production bacteria strains to produce fucosylated oligosaccharides.SOLUTION: The invention provides a nucleic acid construct comprising an isolated nucleic acid encoding a lactose-utilizing α(1,2)fucosyltransferase enzyme. Also provided is a host bacteria production strain comprising Eschericia coli K12 comprising the nucleic acid construct. Further provided is a method for producing a fucosylated oligosaccharide, comprising: culturing the bacterium in the presence of lactose; and retrieving a fucosylated oligosaccharide from the bacterium or from a culture supernatant of the bacterium.SELECTED DRAWING: Figure 2
Inventors:
MATTHEW IAN HEIDTMAN
MASSIMO MERIGHI
JOHN M MCCOY
MASSIMO MERIGHI
JOHN M MCCOY
Application Number:
JP2018018760A
Publication Date:
June 21, 2018
Filing Date:
February 06, 2018
Export Citation:
Assignee:
GLYCOSYN LLC
International Classes:
C12N15/09; C07H3/06; C12N1/21; C12P19/44; C12Q1/48; G16B35/00
Domestic Patent References:
| JP4048173B2 | 2008-02-13 |
Other References:
"Database Genbank [online], Bacteroides vulgatus ATCC 8482 chromosome, complete genome, Accession No.", 2011.11.16, INTERNET: , RE, JPN6016045195, ISSN: 0004167283
Attorney, Agent or Firm:
Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto