To advantageously amplify a nucleic acid by using a polyamide-nucleic acid derivative/DNA primer having a nucleoside unit having 3'-hydroxyl group at one terminal, a thermostable polymerase enzyme and a usually required component.
One or more polyamide-nucleic acid derivatives (PNA)/DNA primers having at least one nucleoside unit having 3'-hydroxyl group at one terminal, a DNA polymerase derived from a microorganism such as the genus Thermus, Thermococcus or Pyrococcus as a thermostable DNA polymerase and a usually required component are used and reacted according to a method similar to a well-known amplifying method such as a polymerase chain reaction(PCR) and a ligase chain reaction (LCR) to thereby advantageously amplify the nucleic acid according to the method usable in diagnosis of viral infection or diseases or cancer diagnosis, etc.
BREIPOHL GERHARD DR
BRENNER STEVEN A PROF DR
LUTZ MICHAEL
