AMPLIFICATION USING MODIFIED PRIMER - F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT

Title:

AMPLIFICATION USING MODIFIED PRIMER

Document Type and Number:

Japanese Patent JP3737734

Kind Code:

B2

Abstract:

PROBLEM TO BE SOLVED: To inhibit the formation of a template-nondependent nonspecific product (primer-dimer) in a nucleic acid amplification reaction.
SOLUTION: A set of primer pair is used for inhibiting the formation of a template-nondependent nonspecific product (primer-dimer) in a nucleic acid amplification reaction, wherein, at least one primer thereof includes a nucleotide modified at three 3'-terminal nucleotide positions, and the modified nucleotide is selected from the group consisting of 2'-O-methylnucleotides, 2'- fluoronucleotides, 2'-aminonucleotides and arabinose nucleotides.

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Inventors:

Walter Jay Rayard
John Tea. Neemeek

Application Number:

JP2001326463A

Publication Date:

January 25, 2006

Filing Date:

October 24, 2001

Export Citation:

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Assignee:

F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT

International Classes:

C12N15/09; C12Q1/68; C12Q1/6848; C12Q1/6853; (IPC1-7): C12N15/09; C12Q1/68

Domestic Patent References:

JP2002119291A
JP2002191372A

Foreign References:

US6001611
WO2000056747A1
WO1998002582A1

Other References:

Nucleic Acids Research, 23[11](1995) p.2019-2024
Nucleic Acids Research, 22[ 1](1994) p.20-24
Nucleic Acids Research, 28[18](2000) p.3625-3635

Attorney, Agent or Firm:

Takashi Ishida
Jun Tsuruta
Yoichi Watanabe
Masaya Nishiyama
Higuchi Souji

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