Title:
Ｔ細胞受容体およびＢ細胞受容体レパトアの解析システムならびにその治療および診断への利用
Document Type and Number:
Japanese Patent JP6661106
Kind Code:
B2
Abstract:
The repertoire of the variable region of T cell receptors (TCR) or B cell receptors (BCR) is quantitatively analyzed using non-biased gene sequence analysis. The present invention provides the following: a method for quantitatively analyzing the repertoire of the variable region of the T cell receptors (TCR) or B cell receptors (BCR) of a subject by using a database, wherein the method includes (1) a step for providing a nucleic acid sample containing the nucleic acid sequence of T cell receptors (TCR) or B cell receptors (BCR) amplified in a non-biased manner from the subject; (2) a step for determining the nucleic acid sequence contained in the nucleic acid sample; and (3) a step for calculating the frequency of appearance of each gene or combination thereof on the basis of the determined nucleic acid sequence and deriving the TCR or BCR repertoire of the subject.
Inventors:
Ryuji Suzuki
Arai
Arai
Application Number:
JP2017118613A
Publication Date:
March 11, 2020
Filing Date:
June 16, 2017
Export Citation:
Assignee:
repertoire genesis Co., Ltd.
International Classes:
C12Q1/6869; A61K35/17; A61K38/02; A61K39/00; A61P35/00; C12M1/00; C12N15/12; C12Q1/6851; G16B30/00; G16B30/10
Domestic Patent References:
| JP2012508011A | ||||
| JP2013524849A |
Foreign References:
| WO2013134162A2 |
Other References:
454 Sequencing System: Guidelines for Amplicon Experimental Design,2012年,pp.1-54
Scand. J. Immunol.,2006年,vol.64, no.1,pp.53-60
Nucleic Acids Res.,1990年,vol.18, no.14,pp.4293-4294
Br. J. Haematol.,2000年,vol.109, no.4,pp.759-769
Genome Med.,2013年10月,vol.5, no.10,pp.98(1-13)
Genome Res.,2009年,vol.19, no.10,pp.1817-1824
Eur. J. Immunol.,2012年,vol.42, no.11,pp.3073-3083
J. Immunol. Methods,2012年,vol.384, no.1-2,pp.81-91
Scand. J. Immunol.,2006年,vol.64, no.1,pp.53-60
Nucleic Acids Res.,1990年,vol.18, no.14,pp.4293-4294
Br. J. Haematol.,2000年,vol.109, no.4,pp.759-769
Genome Med.,2013年10月,vol.5, no.10,pp.98(1-13)
Genome Res.,2009年,vol.19, no.10,pp.1817-1824
Eur. J. Immunol.,2012年,vol.42, no.11,pp.3073-3083
J. Immunol. Methods,2012年,vol.384, no.1-2,pp.81-91
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takeshi Komatani
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Natsuki Morishita
Takeshi Komatani
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto