Title:
ANTI-2'-DEOXYCYTIDINE ANTIBODY, AND MANUFACTURE AND USE THEREOF
Document Type and Number:
Japanese Patent JP3849001
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide an excellent antibody having high specificity for 2'- deoxycytidine(dCyd) by transforming the 5-th or 3rd position hydroxyl group of dcyd into succinyl, dehydration-linking it with the amino group of carrier polypeptide via the carboxyl group, and using the dCyd antigen expressed by a formula.
SOLUTION: 2'-deoxycytidine(dCyd) is transformed into succinyl by succinic anhydride, succinylated derivative at the fifth or third position is separated by HPCL, for example, and it is linked with carrier polypeptide or protein to prepare the dCyd antigen expressed by the formula I or II. An animal (mouse, cattle, horse) is immunized against this antigen, antibody forming cells are collected from the immunized animal and cell-fused with myeloma cells to form hybridoma, and an anti-dCyd monoclonal antibody is obtained. The dCyd in a specimen is reacted to the anti-dCyd antibody serving as a reagent, and the existence or abundance of dCyd in the specimen is detected from the reaction result.
Inventors:
Yoshinori Masanori
Application Number:
JP23044296A
Publication Date:
November 22, 2006
Filing Date:
August 30, 1996
Export Citation:
Assignee:
Advanced Life Science Institute Co., Ltd.
Yoshinori Masanori
Yoshinori Masanori
International Classes:
G01N33/53; C07K14/76; C07K16/00; C07K16/44; C12N5/10; C12N15/02; C12P21/08; G01N33/577; (IPC1-7): G01N33/53; C07K16/44; C12N5/10; C12N15/02; C12P21/08; G01N33/577
Domestic Patent References:
| JP5273209A |
Other References:
Clin. Chim. Acta.,1995年,Vol.234,p37-45
J.Immunol. Method.,1984年,Vol.75, No.2,p241-246
J.Immunol. Method.,1984年,Vol.75, No.2,p241-246
Attorney, Agent or Firm:
Takashi Ishida
Fukumoto product
Masaya Nishiyama
Fukumoto product
Masaya Nishiyama