NEW MATERIAL:The thermally denaturated deoxyribonucleic acid PD-01 having antitumor activity developed by thermal denaturation. The sodium salt of the substance has the following physical and chemical properties: appearance, white powder; melting point, no distinct melting point; elementary analysis, C 27.31W 31.44%, H 4.13W5.79%, N 11.03W13.55%, P 6.81W8.22%, Na 3.80W4.81%; molecular weight, 30,000W1,000,000; solubility, soluble in water, insoluble in ethanol, methanol, ether and acetone; base composition (%), guanine 30.0, adenine 19.9, cytosine 30.7, thymine 19.4; effect of enzymatic treatment, loses antitumor activity with deoxyribonuclease I but keeps the activity with ribonuclease T2; etc.
USE: An antitumor agent exhibiting antitumor activity via the immune reaction of the host.
PROCESS: The disintegrated cell of bacteria belonging to Pseudomonas genus is centrifuged, and the resultant nucleic acid fraction is heated at 80W120°C.
MAKIGUCHI NOBUYOSHI
KURAMOTO ETSUROU
YAMAMOTO HIROSHI
OKUYA HIROAKI
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