Title:
ARTIFICIAL RIBONUCLEASE AND ITS PRODUCTION
Document Type and Number:
Japanese Patent JP3658697
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain an artificial ribonuclease capable of cleaving substrate RNAs at specified positions in a certain and selective way, and useful in molecular biology, biological engineering, pharmacology and the like by binding a polyethylene polyamine residue to a midway site of a DNA oligomer, through amide linkage.
SOLUTION: This artificial ribonuclease is obtained by binding a polyethylene polyamine residue to a midway site of a DNA oligomer, through amide linkage and has the structure of formula I (R' is an amide residue of polyethylene polyamine; (n) is an integer of 1-3; B is a base residue of a nucleic acid constituting DNA). During DNA synthesis, a new phosphoroamidite monomer of formula II (R is an ester residue; DMT is an protective group for a hydroxyl group) is reacted to produce an oligonucleotide of formula III (B' is a base residue, whose functional groups are protected, of a nucleic acid constituting DNA) and the ester residue of the resulting oligonucletide is subjected to aminolysis with polyethylene polyamine to obtain the objective ribonuclease.
Inventors:
Makoto Komiyama
Application Number:
JP493297A
Publication Date:
June 08, 2005
Filing Date:
January 14, 1997
Export Citation:
Assignee:
Japan Science and Technology Agency
International Classes:
C12N15/09; C07F9/24; C07H21/04; C12N9/22; (IPC1-7): C12N9/22; C07F9/24; C12N15/09
Domestic Patent References:
| JP7255483A |
Other References:
Tetrahedron Lett., 1994, Vol.35, No.32, pages 5879-82
J. Biochem., 1994, Vol.116, No.4, pages 719-20
J. Biochem., 1994, Vol.116, No.4, pages 719-20
Attorney, Agent or Firm:
Hiroshi Tanaka
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