Title:
ASSAY METHOD FOR MDV-1
Document Type and Number:
Japanese Patent JP2016052307
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide methods for quantifying vaccine and/or virulent strains of Marek's Disease Virus Serotype-1 (MDV-1) in samples derived from birds.SOLUTION: A method comprises the steps of: (i) providing a biological sample from the bird and optionally isolating nucleic acid from the biological sample; (ii) subjecting the biological sample of (i) to real-time quantitative PCR (qPCR) comprising (a) amplifying a region of the pp3B gene within the nucleic acid sample of (i), the region containing a consistent single nucleotide polymorphism (SNP) difference between vaccine and virulent strains of MDV-1, and contacting the amplified nucleic acid of (a) with a detectable nucleic acid probe specific for the SNP of the vaccine strain of MDV-1 and/or a detectable nucleic acid probe specific for the SNP of the virulent strain of MDV-1; and (iii) measuring changes in the detectable signal produced by the probe of (ii). Methods are also provided for the absolute quantification of vaccine and virulent viruses.SELECTED DRAWING: None
Inventors:
SUSAN BAIGENT
VENUGOPAL NAIR
HERVE LE GALLUDEC
VENUGOPAL NAIR
HERVE LE GALLUDEC
Application Number:
JP2015189321A
Publication Date:
April 14, 2016
Filing Date:
September 28, 2015
Export Citation:
Assignee:
ZOETIS W LLC
International Classes:
C12N15/09; C12Q1/68
Other References:
JPN6014045921; Ａｖｉａｎ Ｐａｔｈｏｌｏｇｙ ３６, 2007, ｐ．４６７-４７４
JPN6014045923; Ｊｏｕｒｎａｌ ｏｆ Ｇｅｎｅｒａｌ Ｖｉｒｏｌｏｇｙ ８８, 2007, ｐ．１０８０-１０９６
JPN6014045928; ｑＰＣＲ Ｔｅｃｈｎｉｃａｌ Ｇｕｉｄｅ , 2008, ＳＩＧＭＡ-ＡＬＤＲＩＣＨ
JPN6014045923; Ｊｏｕｒｎａｌ ｏｆ Ｇｅｎｅｒａｌ Ｖｉｒｏｌｏｇｙ ８８, 2007, ｐ．１０８０-１０９６
JPN6014045928; ｑＰＣＲ Ｔｅｃｈｎｉｃａｌ Ｇｕｉｄｅ , 2008, ＳＩＧＭＡ-ＡＬＤＲＩＣＨ
Attorney, Agent or Firm:
Shinjiro Ono
Yasushi Kobayashi
Shigeo Takeuchi
Osamu Yamamoto
Reiko Izumiya
Yasushi Kobayashi
Shigeo Takeuchi
Osamu Yamamoto
Reiko Izumiya