To provide a biochip of high sensitivity and high throughput which fixes an antibody to the surface of a substrate at an arbitrary position without coating the substrate with an adsorption inhibitor, suppresses the adsorption and bonding an unnecessary physiologically active material to a part other than the arbitrary position and can detect a physiologically active material, and its manufacturing method.

In the biochip constituted by fixing the antibody to the surface of the substrate, the surface of the substrate is coated with a polymer having a phosphoryl chlorine group and the antibody is fixed on the polymer.