Title:
生体高分子自動同定方法
Document Type and Number:
Japanese Patent JP4106444
Kind Code:
B2
Abstract:
The invention aims to provide a highly accurate automatic biopolymer determination technique utilizing mass spectrometry whereby calibration prior to measurement or the addition of an internal standard to a sample can be eliminated. The biopolymer automatic identifying method of the invention comprises: retrieving a candidate molecule by matching an observed mass value X obtained by mass spectrometry with a predetermined database; selecting an arbitrary number of candidate molecules with high similarity scores; calibrating the observed mass value X using the candidate molecule as an internal standard; calculating relative error Ec between a calibrated mass value Xc and a theoretical mass value M of the candidate molecule; determining the standard deviation SEc of the relative error; determining a tolerance Tc of database search from the standard deviation SEc; and repeating a database search based on the tolerance Tc.
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Inventors:
Toru Natsume
Hiroshi Nakayama
Hiroshi Nakayama
Application Number:
JP2004534155A
Publication Date:
June 25, 2008
Filing Date:
September 04, 2003
Export Citation:
Assignee:
National Institute of Advanced Industrial Science and Technology
International Classes:
G01N27/62; G01N33/483; H01J49/04
Domestic Patent References:
| JP10132786A |
Other References:
EGELHOFER, V.,ANALYTICAL CHEMISTRY,2000年 7月 1日,V72 N13,P2741-2750
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Naomi Shimamura
Ishii Teiji
Satoshi Fujita
Naomi Shimamura