To obtain a biotissue staining agent capable of staining a biotissue through zinc and cadmium to enable sensitive and specific discrimination by including a specific quinolylformazan derivative.
The objective staining agent contains a 2quinolylformazan derivative of the formula R1 is H or a condensed polycyclic aromatic group; R2 is H, sulfonic acid group, carboxylic acid group or hydroxyl group). The derivative of the formula can be produced e.g. by reacting 2-chloroquinoline with hydrazine to obtain 2-quinolylhydrazine, reacting the product with a condensed polycyclic aromatic aldehyde (e.g. naphthalaldehyde and phenanthraldehyde) in an organic solvent and reacting the reaction product with a phenyldiazonium salt. Zinc and cadmium in a biotissue can be specifically detected and discriminated by the color to enable the determination of the distribution of zinc and cadmium in the biotissue.
TAKATSU AKIKO
UCHIUMI AKIRA
TAKATSU AKIKO
Toshiaki Ikeura