Title:
血液脳関門モデルならびにそれを作製する方法および使用する方法
Document Type and Number:
Japanese Patent JP7075132
Kind Code:
B2
Abstract:
Provided herein is an in vitro model of the blood brain barrier. In some embodiments, the model includes: an endothelial cell layer, and brain tissue layer comprising neuronal cells, and optionally one or more of astrocytes, pericytes, oligodendrocytes, and microglia. In some embodiments, the model further comprises a porous membrane between said endothelial cell layer and the neuronal cell layer. A microfluidic device comprising the same and methods of use thereof are also provided.
Inventors:
Wicks, robert tee
Atara, Anthony
Elephant, goodwell
Wicks, Elizabeth Y
Atara, Anthony
Elephant, goodwell
Wicks, Elizabeth Y
Application Number:
JP2019505398A
Publication Date:
May 25, 2022
Filing Date:
August 04, 2017
Export Citation:
Assignee:
Wake Forest University Health Sciences
International Classes:
C12N5/079; C12M1/00; C12Q1/02; G01N33/15; G01N33/50
Foreign References:
| WO2016100695A1 | ||||
| US20120015395 | ||||
| US20140127800 |
Other References:
Biomicrofluidics,2015年,No.9,Article No.054124
Fluids and Barriers of the CNS,2013年,Vol.10, No.1,Article No.33
PLOS ONE,2016年,Vol.11, No.3,e0150360
Journal of Neurochemistry,2011年,Vol.119, No.3,p.507-520
Scientific Reports,2014年,Vol.4,Article No.04160
Scientific Reports,2013年,Vol.3,Article No.01500
Fluids and Barriers of the CNS,2013年,Vol.10, No.1,Article No.33
PLOS ONE,2016年,Vol.11, No.3,e0150360
Journal of Neurochemistry,2011年,Vol.119, No.3,p.507-520
Scientific Reports,2014年,Vol.4,Article No.04160
Scientific Reports,2013年,Vol.3,Article No.01500
Attorney, Agent or Firm:
Okuyama Shoichi
Tetsuo Matsushima
Nakamura Ayako
Soji Morimoto
Yu Tanaka
Koichi Tokumoto
Akiko Mizushima
Kohei Arihara
Tetsuo Matsushima
Nakamura Ayako
Soji Morimoto
Yu Tanaka
Koichi Tokumoto
Akiko Mizushima
Kohei Arihara