Title:
【発明の名称】融合タンパク質の試験管内プロセシング
Document Type and Number:
Japanese Patent JP3140488
Kind Code:
B2
Abstract:
There is provided a novel method for the production of a biologically active protein comprising treating a fusion protein consisting of 1. one or multiple successive protein segment(s) each consisting of said biologically active protein the C-terminal amino acid of which is joined to a linker polypeptide sequence L and 2. a polypeptide tag joined to the C-terminal amino acid of said successive protein segment(s), or consisting of 1. a polypeptide tag joined to the N-terminal amino acid of 2. multiple successive protein segments each consisting of a linker polypeptide sequence L and said biologically active protein, with soluble yeast endoprotease yscF and with soluble yeast carboxypeptidase ysc alpha , and isolating said biologically active protein.
Inventors:
Yutta Heim
Peter Seebots
Kenji Takabayashi
Peter Seebots
Kenji Takabayashi
Application Number:
JP17655291A
Publication Date:
March 05, 2001
Filing Date:
July 17, 1991
Export Citation:
Assignee:
Novartis Actien Gezel Shaft
International Classes:
A61K38/21; A61K38/22; A61K38/23; A61K38/28; A61K38/46; A61K38/55; A61K39/395; C07K14/005; C07K14/195; C07K14/435; A61K38/00; C07K14/52; C07K14/555; C07K14/56; C07K14/575; C07K14/585; C07K14/65; C07K14/745; C07K14/815; C07K16/00; C07K19/00; C12N1/19; C12N1/21; C12N9/00; C12N9/60; C12N15/09; C12N15/62; C12P21/02; C12P21/06; C12R1/19; C12R1/865; (IPC1-7): C12N15/09; C07K14/56; C07K14/585; C07K14/65; C07K14/745; C07K14/815; C07K19/00; C12N1/19; C12N1/21; C12P21/02
Domestic Patent References:
| JP6485082A | ||||
| JP1124390A |
Other References:
ＦＥＢＳ Ｌｅｔｔｅｒｓ，Ｖｏｌ．２１８［１］（１９８７）ｐ．３１−３４
Attorney, Agent or Firm:
Aoki Akira (3 outside)