Title:
BRANCHED TYPE OLIGONUCLEOTIDE
Document Type and Number:
Japanese Patent JP3771660
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain the subject new oligonucleotide, comprising a branched type oligonucleic acid prepared by a solid-phase phosphoramidite method using a pentaerythritol compound, capable of forming triple strands with various nucleic acids and useful as an antisense molecule, etc.
SOLUTION: This new branched type oligonucleotide is represented by formula I [R1 and R2 are each a DNA or an RNA; (n) is a number of 2-6] and is capable of forming a triple-stranded nucleic acid with various DNAs or RNAs and thereby useful as an antisense molecule for gene therapy, etc., according to an antisense method. The branched type oligonucleotide is readily obtained by synthesizing oligonucleotide chains in four directions according to a solid- phase phosphoramidite method using a pentaerythritol compound which is a new compound represented by formula II [Tr is a (substituted)trityl; A is a phosphate group, a phosphonate group or phosphoramidite group].
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Inventors:
Akira Matsuda
Yoshihito Ueno
Yoshihito Ueno
Application Number:
JP5593797A
Publication Date:
April 26, 2006
Filing Date:
March 11, 1997
Export Citation:
Assignee:
Taiho Pharmaceutical Co., Ltd.
Akira Matsuda
Akira Matsuda
International Classes:
C07H21/02; C12N15/09; A61K31/70; C07F9/09; C07F9/22; C07F9/40; C07H21/04; A61K31/7052; (IPC1-7): C07H21/02; C07F9/09; C07F9/22; C07F9/40; C07H21/04; C12N15/09; //A61K31/70
Other References:
von Buren, M. et al.,Branched Oligodeoxynucleotides: Automated Synthesis and Triple Helical Hybridization Studies,Tetrahedron,1995年,Vol.51, No.31,pages 8491-8506
Attorney, Agent or Firm:
Miyuki Ariga
Toshio Takano
Toshio Nakajima
Hiromi Matoba
Toshio Takano
Toshio Nakajima
Hiromi Matoba