Title:
BREEDING STRAIN OF BIFIDUS BACTERIA HAVING EXCELLENT PRESERVATION STABILITY AND DRIED BACTERIAL CELL POWDER
Document Type and Number:
Japanese Patent JP3133437
Kind Code:
B2
Abstract:
PURPOSE: To obtain the subject breeding strain having excellent resistance and useful for food, etc., in high yield of survived cell number by culturing bifidus bacteria in a liquid medium, freeze-drying in the absence of a dispersion medium and selecting a strain having high survival rate at a prescribed temperature.
CONSTITUTION: Bifidus bacterial strain such as Bifidobacterium longum WB1001, Bifidobacterium brebe WB1002 and Bifidobacterium bifidum WB1003 is cultured in a liquid medium and freeze-dried in the absence of dispersion medium. The cells having a survival rate of 1×10-1 to 1×10-6% after the storage at 50°C are separated. The operation is repeated several to several tens times to obtain the objective breeding strain.
Inventors:
market price
Nobuyuki Suzuki
Haruhisa Hirata
Yoshitami Ohashi
Masayoshi Goto
Nobuyuki Suzuki
Haruhisa Hirata
Yoshitami Ohashi
Masayoshi Goto
Application Number:
JP33930191A
Publication Date:
February 05, 2001
Filing Date:
November 29, 1991
Export Citation:
Assignee:
Wakamoto Pharmaceutical Co., Ltd.
International Classes:
A23L1/30; A23L33/10; A23L33/135; A61K35/74; A61K35/745; A61P1/00; A61P1/12; C12N1/20; (IPC1-7): C12N1/20; A23L1/30
Domestic Patent References:
| JP6322179A |