Title:
褐色脂肪細胞上清、その調製法、及び、使用
Document Type and Number:
Japanese Patent JP7465506
Kind Code:
B2
Abstract:
The present invention provides a composition having a metabolism-improving action, which comprises a supernatant of brown adipocytes or a purified product thereof. The present invention also provides a method of preparing the supernatant without using a culture solution comprising a high concentration of glucose. The present invention also provides a method of producing brown adipocytes using pluripotent stem cells, which are useful for preparing the supernatant of brown adipocytes. The present invention has succeeded in obtaining a supernatant having a metabolism-improving action from brown adipocytes. It was also possible to obtain the supernatant without using a culture solution comprising a high concentration of glucose. The present invention has also succeeded in producing brown adipocytes from pluripotent stem cells using a feeder-free culture system without adding a cytokine cocktail or the like. The brown adipocyte supernatant of the present invention is expected to be applied to the development of therapeutic agents and cosmetic products for glucose metabolism diseases.
Inventors:
Kumiko Saeki
Norihiko Kobayashi
Masako Oka
Kazunori Matsumura
Miwako Nishio
Zhu Afeng
Toyotaka Mori
Norihiko Kobayashi
Masako Oka
Kazunori Matsumura
Miwako Nishio
Zhu Afeng
Toyotaka Mori
Application Number:
JP2020537087A
Publication Date:
April 11, 2024
Filing Date:
August 14, 2019
Export Citation:
Assignee:
National Center for International Medical Research
Id Pharma Co., Ltd.
Id Pharma Co., Ltd.
International Classes:
A61K35/35; A61K8/98; A61P3/00; A61P3/04; A61P3/10; A61P5/50; A61P17/00; A61P43/00; A61Q19/00
Domestic Patent References:
| JP2018108080A |
Foreign References:
| WO2012147853A1 | ||||
| WO2013003595A1 |
Other References:
NISOLI, E., et al.,Expression of Nerve Growth Factor in Brown Adipose Tissue: Implications for Thermogenesis and Obesit,Endocrinology, 1996, Vol.137, No.2, pp.495-503,Abstract, 第496頁, 第502頁最終段落
Journal of Biological Chemistry,2011年,Vol.286, No.40,pp.34552-34558
NISHIO, M., et al.,Production of Functional Classical Brown Adipocytes from Human Pluripotent Stem Cells using Specific,Cell Metabolism,2012年,Vol.16,pp.394-406
佐伯久美子,ヒト多能性幹細胞からの褐色脂肪細胞の作製,医学のあゆみ,2014年,Vol.250, No.9,pp.816-821
佐伯久美子,ヒトES/iPS細胞から樹立した褐色脂肪細胞の機能,The Lipid,2014年,Vol.25, No.1,pp.74-80
Journal of Biological Chemistry,2011年,Vol.286, No.40,pp.34552-34558
NISHIO, M., et al.,Production of Functional Classical Brown Adipocytes from Human Pluripotent Stem Cells using Specific,Cell Metabolism,2012年,Vol.16,pp.394-406
佐伯久美子,ヒト多能性幹細胞からの褐色脂肪細胞の作製,医学のあゆみ,2014年,Vol.250, No.9,pp.816-821
佐伯久美子,ヒトES/iPS細胞から樹立した褐色脂肪細胞の機能,The Lipid,2014年,Vol.25, No.1,pp.74-80
Attorney, Agent or Firm:
Masao Haruna
Hatsushi Shimizu
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto
Hatsushi Shimizu
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto