Title:
がん細胞の培養方法
Document Type and Number:
Japanese Patent JP7213487
Kind Code:
B2
Abstract:
To provide a method for culturing a cancer cell in which spheroid of a cancer cell in blood can be formed at higher probability than conventional ones.SOLUTION: A method for culturing cancer cell includes: a process of capturing a cancer cell in blood on a hydrophilic polymer layer; a process of peeling the cancer cell captured on the hydrophilic polymer layer by enzyme for cell dissociation; a process of performing centrifugal separation to solution including the peeled cancer cell at the centrifugal force of 100 to 1000G; and a process of culturing the cancer cell on an artificial basement membrane by ES cell culture medium.SELECTED DRAWING: None
Inventors:
Yasuhisa Minagawa
Hirofumi Yamamoto
Naoto Haraguchi
Masaki Mori
Hirofumi Yamamoto
Naoto Haraguchi
Masaki Mori
Application Number:
JP2019041520A
Publication Date:
January 27, 2023
Filing Date:
March 07, 2019
Export Citation:
Assignee:
Sumitomo Rubber Industries, Ltd.
National University Corporation Osaka University
National University Corporation Osaka University
International Classes:
C12N5/09
Domestic Patent References:
| JP2008050548A | ||||
| JP2008011797A |
Foreign References:
| WO2018169007A1 | ||||
| WO2017155869A1 | ||||
| CN106854639A | ||||
| WO2016125884A1 |
Attorney, Agent or Firm:
Patent Attorney Firm WisePlus