NEW MATERIAL:A carcinostatic substance TF-340 and a salt thereof. Appearance: Whitish gray W light brown powder. Melting point: Indefinite, begins to decompose at about 140°C and remarkably decomposes at 200°C or above. Physiological actions: Capable of inhibiting the multiplication of murine Ehrlich ascitic cancers, and having the immunological activating action. Soluble in water and insoluble in methanol, ethanol, etc. Color reactions: Positive to the Molisch, phenol-sulfuric acid, anthrone-sulfuric acid reactions, etc. and negative to the ninhydrin reaction.
USE: A carcinostatic agent characterized by the slight side effects thereof.
PROCESS: A microorganism, e.g. Fusobacterium nucleatum TF-031 strain, of the genus Fusobacterium is cultivated under anaerobic conditions, and a hydrophilic organic solvent is then added to the resultant supernatant liquid. The resultant fraction having the carcinostatic action of the formed precipitate is then treated with a proteolytic enzyme to collect the fraction of the aimed carcinostatic substance TF-340.
SAIKAWA ISAMU
YASUDA TAKASHI
MURAKAMI SHIYOUHACHI
MAEDA TOYOO
TSUDA HISATSUGU
SAKAI HIROSHI
SUGITA MASATOSHI
YAMAMOTO YOSHIKO
MINAMI TAKASHI
HORI TAKAKO
Next Patent: CARCINOSTATIC SUBSTANCE TF-350, ITS PREPARATION AND CARCINOSTATIC AGENT CONTAINING THE SAME