Title:
CELL CULTURE METHOD, PARTICULATE CULTURE CARRIER, AND PARTICLE-CONTAINING CELL AGGREGATE
Document Type and Number:
Japanese Patent JP2014226097
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a culture method which can maintain cellular functions by improving permeability of nutrition, oxygen, a drug and a reagent from culture medium, while adhering a three-dimensional aggregate with the cellular function similar to organisms on a culture vessel.SOLUTION: A cell aggregate containing particles can be formed while adhering cells to a culture vessel by giving micro scaffold particles having cell adhesion and substance permeability on the cell after plating isolated cells in the culture vessel and sedimenting the cells on a culture surface. For example, the cell aggregate containing particles is formed by giving gelatin particles with a size of 1/5 or more of a cell diameter, as a culture carrier contained in the cell aggregate, at the rate of a cell number: particle number is 80:1 or more based on the number of the cultured cells per unit culture area.
Inventors:
HISADA AKIKO
SONODA HIROSHI
SONODA HIROSHI
Application Number:
JP2013109154A
Publication Date:
December 08, 2014
Filing Date:
May 23, 2013
Export Citation:
Assignee:
HITACHI HIGH TECH CORP
International Classes:
C12N1/00; C12M1/00; C12M3/00; C12N5/07
Domestic Patent References:
| JP2011004612A | 2011-01-13 | |||
| JP2004275122A | 2004-10-07 | |||
| JPH078273A | 1995-01-13 |
Foreign References:
| WO2011059112A1 | 2011-05-19 | |||
| WO2010079602A1 | 2010-07-15 | |||
| WO2007037407A1 | 2007-04-05 |
Attorney, Agent or Firm:
Polaire Intellectual Property Corporation
Previous Patent: METHOD FOR PRODUCING PLANT CUTTING SEEDLING
Next Patent: HYDROPONIC APPARATUS AND HYDROPONIC METHOD
Next Patent: HYDROPONIC APPARATUS AND HYDROPONIC METHOD