Title:
細胞培養方法
Document Type and Number:
Japanese Patent JP6628416
Kind Code:
B2
Abstract:
Provided is a technique for obtaining a fibrous protein material in which cells are embedded at a high density, and with which it is possible to: make an extracellular matrix component contact the cells discretionarily; control the three-dimensional form; maintain the shape of a tissue after hydrogel removal; efficiently proliferate cells in a tissue; form intercellular interaction; and uniformly inseminate cells into a tissue. This method for producing a fibrous protein material comprises: continuously introducing a first aqueous solution in which sodium alginate and a protein have been dissolved and a gelling agent aqueous solution to a flow path structure that includes an inlet to which the first aqueous solution is introduced, an inlet to which the gelling agent aqueous solution is introduced, inlet flow paths connected to the respective inlets, a merging flow path where the inlet flow paths merge, and an outlet downstream of the merging flow path; forming a hydrogel by continuously gelling the first aqueous solution inside the flow path structure; and chemically crosslinking the protein included in the hydrogel outside or inside the flow path structure, and removing alginic acid included in the hydrogel.
Inventors:
Masumi Yamada
Minoru Seki
Ayaka Hori
Yu Hirai
Yuya Yajima
Yoichi Kitagawa
Minoru Seki
Ayaka Hori
Yu Hirai
Yuya Yajima
Yoichi Kitagawa
Application Number:
JP2016540188A
Publication Date:
January 08, 2020
Filing Date:
July 31, 2015
Export Citation:
Assignee:
National University Corporation Chiba University
International Classes:
D01F4/00; C08H1/00; C12N5/07; D01D5/06
Domestic Patent References:
| JP2013009598A |
Foreign References:
| WO2011046105A1 |
Other References:
Hiroaki Onoe et al.,Metre-long cell-laden microfibres exhibit tissue morphologies and functions,nature materials,英国,Nature Publishing Group,2013年 3月31日,12,584-590,特に584-586頁、supplimentary informationの4,5頁