Title:
生理的イオン強度でタンパク質を吸着させるためのクロマトグラフィー材料
Document Type and Number:
Japanese Patent JP4801598
Kind Code:
B2
Abstract:
Ion exchange and hydrophobic interaction chromatographic materials are constructed by tethering a terminal binding functionality to a solid support via a hydrophobic linker. The backbone of the linker typically comprises sulfur-containing moieties. Suitable terminal binding functionalities are tertiary amines, quaternary ammonium salts, or hydrophobic groups. These chromatographic materials possess both hydrophobic and ionic character under the conditions prescribed for their use. The separation of proteins from crude mixtures at physiological ionic strength can be accomplished with a chromatographic material of this type by applying pH or ionic strength gradients, thereby effecting protein adsorption and desorption.
Inventors:
Bossetti, Egist
Guillott, Pierre
Guillott, Pierre
Application Number:
JP2006551171A
Publication Date:
October 26, 2011
Filing Date:
January 14, 2005
Export Citation:
Assignee:
Pall Corporation
International Classes:
G01N30/88; B01J20/289; C07K1/16; C07K1/18; C07K1/20; G01N27/62; G01N30/72; G01N33/543; G01N33/68; B01D15/32; B01D15/36; G01N30/02; G01N35/00
Domestic Patent References:
| JP10506987A | ||||
| JP10502339A | ||||
| JP2000502951A |
Attorney, Agent or Firm:
Yuichi Yamada
Masakazu Noda
Kazuhiro Yamaguchi
Ikeda adult
Hiroto Kido
Masato Ikeda
Masakazu Noda
Kazuhiro Yamaguchi
Ikeda adult
Hiroto Kido
Masato Ikeda