Title:
CLADIMEA TRACHOMA STRAIN SPECIFIC ANTIGEN AND MANUFACTURE
Document Type and Number:
Japanese Patent JPS57158725
Kind Code:
A
Abstract:
Procedures are presented for isolating the major outer membrane protein of Chlamydia trachomatis. The isolated protein is a species specific antigen which comprises about 60% of the C. trachomatis cell outer membrane structure. The protein has a molecular weight ranging from about 38,000 to 44,000 daltons, with a mean molecular weight of about 39,500 daltons. The protein antigen is purified from C. trachomatis cells by first extracting the cell contents with a mild anionic detergent, preferably sarcosyl, to leave a residue of intact outer cell membranes. These outer cell membranes are then extracted with a strong anionic detergent, preferably sodium dodecyl sulfate, which solubilizes the 39,500 dalton antigen. The antigen is then purified by hydroxylapatite chromatography. The antigen is species specific for Chlamydia trachomatis and may be utilized in assaying Chlamydial infection in mammals.
Inventors:
HAARAN DEII KARUDOUERU
JIYURIUSU SHIYACHITAA
JIYURIUSU SHIYACHITAA
Application Number:
JP3251782A
Publication Date:
September 30, 1982
Filing Date:
March 03, 1982
Export Citation:
Assignee:
UNIV CALIFORNIA
International Classes:
A61K39/118; C07K14/005; C07K14/195; C07K14/295; C07K14/41; C07K16/12; G01N33/569; A61K38/00; A61K39/00; (IPC1-7): A61K39/118; A61K39/395
Other References:
JOURNAL OF GENERAL MICROBIOLOGY=1981
IMMUNOLOGIC DISEASES OF THE MUCOUS MEMBRANES=1980
JOURNAL OF BACTERIOLOGY=1974
BIOCHIMICA ET BIOPHYSICA ACTA=1977
JOURNAL OF BACTERIOLOGY=1973
MICROBIOLOGICAL REVIEWS=1978
JOURNAL OF BACTERIOLOGY=1967
IMMUNOLOGIC DISEASES OF THE MUCOUS MEMBRANES=1980
JOURNAL OF BACTERIOLOGY=1974
BIOCHIMICA ET BIOPHYSICA ACTA=1977
JOURNAL OF BACTERIOLOGY=1973
MICROBIOLOGICAL REVIEWS=1978
JOURNAL OF BACTERIOLOGY=1967