PURPOSE: To readily obtain a highly unsaturated fatty acid in high yield by selectively hydrolyzing phosphatidyl ethanolamine contained in phospholipid of egg yolk with phospholipase A2 derived from swine pancreas.
CONSTITUTION: Phospholipid of egg yolk is fractionated to give phosphatidyl ethanolamin (A) of egg yolk comprising 16-18C fatty acid having 0-20 unsaturation degree. Then, 1 pt.wt. component A is dissolved in 10-500 pts.wt. organic solvent such as diethyl ether to give a solvent solution (B). Then phospholipase A2 (C) is added to the solution B in an amount to give 0.1-100mg phospholipase A2 based on 1g component A to give a reaction solution (D). Then the component D is reacted at 30-60°C for about 3 hours with stirring at 60-150rpm while adjusting to a given pH with Ca salt, etc., to give a reaction product (E). Then the component H is washed with an ether, purified by reprecipitation with acetone to collect highly unsaturated fatty acids such as arachidonic acid and docosahexaenoic acid.
FUKUDA NOBUO
NAKACHI OSAMU
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