Title:
大腸上皮幹細胞の単離・培養技術と、これを用いた大腸上皮移植技術
Document Type and Number:
Japanese Patent JP6544726
Kind Code:
B2
Abstract:
It is an object of the present invention to provide a culture medium for in vitro culture of a colorectal epithelial stem cell or the like, a method of in vitro culturing a colorectal epithelial stem cell or the like using the culture medium, a prophylactic or therapeutic agent for a bowel disease containing a colorectal epithelial stem cell or the like cultured by the method, a method of administrating, i.e., transplanting a colorectal epithelial stem cell or the like cultured by the method to a bowel disease patient, and a method of isolating a colorectal epithelial stem cell or the like. The culture medium for in vitro culture of a colorectal epithelial stem cell and/or a colorectal epithelial cell characteristically contains serum albumin, Wnt3a, and r-spondin-1.
Inventors:
Mamoru Watanabe
Tetsuya Nakamura
Tetsuya Nakamura
Application Number:
JP2017208883A
Publication Date:
July 17, 2019
Filing Date:
October 30, 2017
Export Citation:
Assignee:
Tokyo Medical And Dental University, Medical Hospital
International Classes:
C12N5/071; A61K35/38; A61L27/38; A61P1/00; A61P1/04
Foreign References:
| WO2010090513A1 | ||||
| US20040175367 | ||||
| US20060073591 |
Other References:
JUNG P. et al.,Nature Medicine, 04 September 2011, Vol.17, No.10, pp.1225-1227 and Supplementary information
SATO T. et al.,Gastroenterology, 05 September 2011, Vol.141, pp.1762-1772
Materials, 2010, Vol.3, pp.1863-1887, DOI: 10.3390/ma3031863
SATO T. et al.,Nature, 2009, Vol.459, pp.262-265
Journal of Pharmacology and Experimental Therapeutics,2003, Vol.307, No.1, pp.146-151
WEIGMANN B. et al.,Nature Protocols, 2007, Vol.2, No.10, pp.2307-2311
LIU S. et al.,Reproductive Biology and Endocrinology, 24 October 2011, Vol.9, No.141, pp.1-9
CHE X. et al.,In Vitro Cellular & Developmental Biology - Animal, 21 June 2011, Vol.47, pp.454-458
Laboratory Investigation,2003, Vol.83, No.7, pp.949-962
American Journal of Physiology - Gastrointestinal and Liver Physiology,2007, Vol.293, pp.G230-G239
SATO T. et al.,Gastroenterology, 05 September 2011, Vol.141, pp.1762-1772
Materials, 2010, Vol.3, pp.1863-1887, DOI: 10.3390/ma3031863
SATO T. et al.,Nature, 2009, Vol.459, pp.262-265
Journal of Pharmacology and Experimental Therapeutics,2003, Vol.307, No.1, pp.146-151
WEIGMANN B. et al.,Nature Protocols, 2007, Vol.2, No.10, pp.2307-2311
LIU S. et al.,Reproductive Biology and Endocrinology, 24 October 2011, Vol.9, No.141, pp.1-9
CHE X. et al.,In Vitro Cellular & Developmental Biology - Animal, 21 June 2011, Vol.47, pp.454-458
Laboratory Investigation,2003, Vol.83, No.7, pp.949-962
American Journal of Physiology - Gastrointestinal and Liver Physiology,2007, Vol.293, pp.G230-G239
Attorney, Agent or Firm:
Masaki Hirota
Seiji Ozawa
Yusaku Tokai
Makoto Horiuchi
Seiji Ozawa
Yusaku Tokai
Makoto Horiuchi