Title:
組合せ抗体ライブラリー及びその使用
Document Type and Number:
Japanese Patent JP5882058
Kind Code:
B2
Abstract:
Methods for making a combinatorial antibody library from human germline segments are provided. Also provided are libraries of nucleic acid molecules compiled from germline segments encoding VL chains and libraries of nucleic acid molecules encoding VH chains, and resulting antibody libraries. The libraries are provided as addressable libraries. Methods for screening antibody libraries against a target protein antigen, and the identified or selected antibodies are provided.
Inventors:
Bone smider
James Graziano
Helen Hong Yuan Mao
Byung Du Sung
Tyson chase
Omar Basil Gun
James Graziano
Helen Hong Yuan Mao
Byung Du Sung
Tyson chase
Omar Basil Gun
Application Number:
JP2011535649A
Publication Date:
March 09, 2016
Filing Date:
November 04, 2009
Export Citation:
Assignee:
Fabras LLC
International Classes:
C40B40/00; C12N15/09; C40B30/00; C40B50/02
Domestic Patent References:
| JP2006518585A | ||||
| JP2005526518A |
Foreign References:
| WO2005023993A1 |
Other References:
LIU, R. et al.,"Combinatorial peptide library methods for immunobiology research.",EXP. HEMATOL.,2003年 1月,Vol.31, No.1,P.11-30
DE WILDT, R.M.T. et al.,"ANTIBODY ARRAYS FOR HIGH-THROUGHPUT SCREENING OF ANTIBODY-ANTIGEN INTERACTIONS.",NATURE BIOTECHNOLOGY,NATURE PUBLISHING GROUP,2000年 9月 1日,Vol.18, No.9,P.989-994
HOOGENBOOM, H.R. et al.,"By-passing immunisation. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro.",JOURNAL OF MOLECULAR BIOLOGY,1992年 9月20日,Vol.227, No.2,P.381-388
SHIN, D.S. et al.,"Combinatorial solid phase peptide synthesis and bioassays.",J. BIOCHEM. MOL. BIOL.,2005年 9月30日,Vol.38, No.5,pp.517-525
DE WILDT, R.M.T. et al.,"ANTIBODY ARRAYS FOR HIGH-THROUGHPUT SCREENING OF ANTIBODY-ANTIGEN INTERACTIONS.",NATURE BIOTECHNOLOGY,NATURE PUBLISHING GROUP,2000年 9月 1日,Vol.18, No.9,P.989-994
HOOGENBOOM, H.R. et al.,"By-passing immunisation. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro.",JOURNAL OF MOLECULAR BIOLOGY,1992年 9月20日,Vol.227, No.2,P.381-388
SHIN, D.S. et al.,"Combinatorial solid phase peptide synthesis and bioassays.",J. BIOCHEM. MOL. BIOL.,2005年 9月30日,Vol.38, No.5,pp.517-525
Attorney, Agent or Firm:
Masutani Takeshi