Title:
二重特異性および多重特異性生物学的物質の区画化されたアッセイ
Document Type and Number:
Japanese Patent JP7257508
Kind Code:
B2
Abstract:
Methods and systems for performing assays in compartmentalized nano-volumes to screen for functional bispecific or multispecific biologics, including: providing a plurality of at least two distinct types of cells, wherein two or more first-type cells are engineered to express substantially a single genetic-variant per cell for a bispecific or multispecific biologic in a secreted form, wherein two or more second-type cells are selected or engineered to produce a positive reporter molecule signal that is triggered by a functional variant of the said biologic expressed by a first-type cell; providing a plurality of compartmentalized nano-volumes, wherein two or more nano-volumes are each provided with substantially one first-type cell, and one or more second-type cell(s); incubating the said nano-volumes over a period of time to allow the expression and secretion of the said biologics inside the said nano-volumes; collecting data representing the positive reporter molecule signal triggered by secreted biologics inside the said nano-volumes, and recovering cells from the nano-volumes with the positive reporter molecule signal and extracting the genetic information representing respective functional variants of the biologics.
Inventors:
George Guikai Wu
Yongley Shan
Yongley Shan
Application Number:
JP2021520203A
Publication Date:
April 13, 2023
Filing Date:
October 07, 2019
Export Citation:
Assignee:
Amberstone Biosciences, Inc.
International Classes:
C12Q1/02; C12Q1/26; C12Q1/6869; G01N33/15; G01N33/50
Domestic Patent References:
| JP2015529826A | ||||
| JP2016533164A |
Foreign References:
| US20160102280 | ||||
| US20050266425 |
Attorney, Agent or Firm:
Yamato Kento
Michiko Matsutani
Yoko Sakurai
Keiji Sakata
Michiko Matsutani
Yoko Sakurai
Keiji Sakata