Title:
歯周硬組織再生用組成物及び歯周硬組織を再生する方法
Document Type and Number:
Japanese Patent JP5191386
Kind Code:
B2
Abstract:
An object of the present invention is to provide technology of using a preadipocyte to restore comprehensively bone, cartilage and periodontal ligament, and cause a periodontal hard tissue to regenerate. By culturing a preadipocyte using a culture medium containing (i) at least one member selected from the group consisting of glycerophosphate and salts thereof, and (ii) at least one member selected from the group consisting of ascorbic acid, derivatives thereof and salts thereof and not containing an adrenal cortical hormone in such an amount that promotes differentiation of a preadipocyte into a periodontal hard tissue cell, differentiation of the cell into a periodontal hard tissue cell is induced efficiently. Furthermore, a scaffold is combined with the preadipocyte cultured with the above-mentioned culture medium to be transplanted into a periodontal tissue that requires regeneration.
Inventors:
Shinya Murakami
Tomoko Hashikawa
Yoshiki Sawa
Matsuyama Akira
Hiroshi Komoda
Tomoko Hashikawa
Yoshiki Sawa
Matsuyama Akira
Hiroshi Komoda
Application Number:
JP2008536349A
Publication Date:
May 08, 2013
Filing Date:
September 20, 2007
Export Citation:
Assignee:
National University Corporation Osaka University
International Classes:
C12N5/0775; A61K35/12; A61K35/35; A61L27/00; A61L27/12; A61L27/22; A61L27/38; A61P1/02; A61P21/00; C12N5/07; C12N5/074; C12N5/077
Other References:
JPN6012045510; Ｔｉｓｓｕｅ Ｅｎｇｉｎｅｅｒｉｎｇ Ｖｏｌ．１０， Ｎｏ．３／４, 2004, ｐ．３８１-３９１
JPN6012045513; Ｃｅｌｌ ｂｉｏｌｏｇｙ ｉｎｔｅｒｎａｔｉｏｎａｌ Ｖｏｌ．１９， Ｎｏ．１２, 1995, ｐ．９７９-９８５
JPN6012045513; Ｃｅｌｌ ｂｉｏｌｏｇｙ ｉｎｔｅｒｎａｔｉｏｎａｌ Ｖｏｌ．１９， Ｎｏ．１２, 1995, ｐ．９７９-９８５
Attorney, Agent or Firm:
Eiji Saegusa
Mutsuko Nakano
Masahito Hayashi
Junya Tanaka
Mutsuko Nakano
Masahito Hayashi
Junya Tanaka