Title:
表面結合オリゴヌクレオチドを化学開裂および脱保護するための組成物および方法
Document Type and Number:
Japanese Patent JP7100069
Kind Code:
B2
Abstract:
Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.
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Inventors:
Shaolin Wu
Randall Smith
Peyton Shah
John M Biale
Wayne N George
Elliott John Lawrence
Jie Mao
Xiao Hai Liu
Randall Smith
Peyton Shah
John M Biale
Wayne N George
Elliott John Lawrence
Jie Mao
Xiao Hai Liu
Application Number:
JP2019571259A
Publication Date:
July 12, 2022
Filing Date:
May 14, 2019
Export Citation:
Assignee:
Illumina Incorporated
Illumina Cambridge Limited
Illumina Cambridge Limited
International Classes:
C12N15/10; C07H19/073; C12N15/11
Foreign References:
| WO2007010251A2 | ||||
| WO2017205336A1 |
Attorney, Agent or Firm:
Michiko Matsutani
Yasushi Ochiai
Yasushi Ochiai