COMPOSITIONS AND METHODS COMPRISING PROTEASE VARIANTS

Title:

COMPOSITIONS AND METHODS COMPRISING PROTEASE VARIANTS

Document Type and Number:

Japanese Patent JP2016028571

Kind Code:

A

Abstract:

PROBLEM TO BE SOLVED: To provide protease variants, compositions comprising protease variants, and methods of using such protease variants and compositions.SOLUTION: An isolated protease variant of a parent protease comprises an amino acid sequence comprising three amino acid substitutions selected from among X024G/R, X053G, X078N, X101N, X128A/S and X217L/Q. The variant has proteolytic activity, and each amino acid position of the variant is numbered by determination by alignment of the amino acid sequence of the variant with a specific amino acid sequence. The variant has enhanced proteolytic activity and/or cleaning activity compared to the parent protease.SELECTED DRAWING: None

Inventors:

JOSHUA ROY BASLER
LUIS GUSTAVO CASCAO-PEREIRA
ESTELL DAVID A
JAMES T KELLIS
ALEXANDER PISARCHIK
AYROOKARAN J POULOSE
DANIEL TORRES-PAZMINO

Application Number:

JP2015147895A

Publication Date:

March 03, 2016

Filing Date:

July 27, 2015

Export Citation:

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Assignee:

DANISCO US INC

International Classes:

C12N15/09; A47L15/42; C11D1/00; C11D3/386; C11D7/42; C12N1/21; C12N9/54; D06F35/00

Domestic Patent References:

JP2005519592A

2005-07-07

Foreign References:

WO2008153925A2	2008-12-18
WO2009149144A2	2009-12-10
WO2009149200A2	2009-12-10

Other References:

GODDETTE,D.W.ET AL.: ""Accession:P29599[gi:267046],Definition:RecName: Full=Subtilisin BL; AltName: Full=Alkaline protease", NCBI ENTREZ PROTEIN[ONLINE];22-SEP-2009 UPLOADED,NCBI,[RETRIEVED ON 19 JANUARY 2015], JPN6015002258, ISSN: 0003329790

Attorney, Agent or Firm:

Yuzo Yamazaki
Toshiaki Akamatsu
Ono Wataru
Okuko Masako
Tadao Naito
Chihiro Imai

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