COMPOSITIONS AND METHODS FOR IMPROVED GLYCOPROTEIN SIALYLATION

Title:

COMPOSITIONS AND METHODS FOR IMPROVED GLYCOPROTEIN SIALYLATION

Document Type and Number:

Japanese Patent JP2015057059

Kind Code:

A

Abstract:

PROBLEM TO BE SOLVED: To provide compositions and methods for producing glycoproteins with enhanced sialylation using sialidases, which are enzymes to remove the terminal sialic acid residue from glycoproteins and glycolipids.SOLUTION: A cell line comprises disrupted expression of at least one chromosomally integrated nucleic acid sequence encoding a non-cytoplasmic sialidase. The amino acid sequence of the sialidase is selected from the group consisting of (a) amino acid sequences at least about 96% identical to an amino acid sequence of a specific sequence and (b) amino acid sequences at least about 81% identical to an amino acid sequence of a specific sequence.

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Inventors:

ZHANG MIN
JAMES S ROSS

Application Number:

JP2014208124A

Publication Date:

March 26, 2015

Filing Date:

October 09, 2014

Export Citation:

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Assignee:

SIGMA ALDRICH CO LLC

International Classes:

C12N5/10; C07K14/47; C12N15/00; C12N15/09; C12N15/113; C12P21/02

Domestic Patent References:

JPH08510133A

1996-10-29

Foreign References:

WO2007102432A1

2007-09-13

Other References:

JPN6013041418; Ｇｌｙｃｏｂｉｏｌｏｇｙ，　１９９７，　ｖｏｌ．７，　ｎｏ．７，　ｐ．９７５-９８６
JPN6013041419; Ｂｉｏｃｈｅｍ．　Ｂｉｏｐｈｙｓ．　Ｒｅｓ．　Ｃｏｍｍｕｎ．，　２００１，　ｖｏｌ．２８０，　ｐ．７２６-７３２

Attorney, Agent or Firm:

Nakajima 淳
Kato Kazu -- full

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