Title:
遺伝子変異を有するポリヌクレオチド配列の簡便検出法
Document Type and Number:
Japanese Patent JP7017246
Kind Code:
B2
Abstract:
A method for detecting a gene mutation(s), the method comprising the steps of: hybridizing a single-stranded circular DNA and a primer with a target polynucleotide containing a first region and a second region adjacent to the 3′-side of the first region and containing a mutation; performing a nucleic acid amplification reaction by rolling circle amplification based on formation of a complex of the target polynucleotide, the primer, and the single-stranded circular DNA; and detecting amplified nucleic acid with a detection reagent. In this method, the single-stranded circular DNA contains a sequence complementary to the first region of the target polynucleotide, a primer-binding sequence adjacent to the 5′-side thereof, and preferably a sequence complementary to a detection reagent-binding sequence. The oligonucleotide primer contains a region having a sequence complementary to the second region of the target polynucleotide, and a region adjacent to the 3′-side thereof and having a sequence complementary to the primer-binding sequence of the single-stranded circular DNA.
Inventors:
Masayasu Kuwahara
Application Number:
JP2018529398A
Publication Date:
February 08, 2022
Filing Date:
June 01, 2017
Export Citation:
Assignee:
Gunma University
International Classes:
C12Q1/6827; C12Q1/6816; C12Q1/6844
Foreign References:
| US20150197804 | ||||
| WO2016152936A1 |
Other References:
Analytical Chemistry,2021年06月27日,Vol.88,p.7137-7144
日本化学会第97春季年会講演予稿集,2017年03月03日,1 C4-36
日本化学会第97春季年会講演予稿集,2017年03月03日,1 C4-36
Attorney, Agent or Firm:
Hidewa Patent Office