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Title:
CREATININE AMIDOHYDROLASE PREPARATION
Document Type and Number:
Japanese Patent JPS61227799
Kind Code:
A
Abstract:

PURPOSE: To obtain a creatinine amidohydrolase (CRN) preparation having remarkably improved enzymatic activity and reduced CRN content in the preparation and storable without causing the clouding of the preparation, by adding bivalent manganese ion to CRN.

CONSTITUTION: The objective preparation can be produced by adding bivalent manganese ion (e.g. manganese chloride, manganese sulfate, manganese acetate, etc.) to CRN originated from microorganism belonging e.g. Alcaligenes genus, Pseudomonas genus, etc. The preparation is compounded preferably further with a nonionic surfactant (preferably a polyoxyethylene alkyl ether having an HLB of ≥10, a polyoxyethylene alkylphenyl ether having an HLB of ≥10, etc.) and a preservative (e.g. azide, antibiotic substance, etc.).


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Inventors:
FUJIMURA KATSUYUKI
ASANO KENJI
INOUE YOSHIO
NAKAMURA AKISHIRO
Application Number:
JP6975285A
Publication Date:
October 09, 1986
Filing Date:
April 01, 1985
Export Citation:
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Assignee:
KOBAYASHI PHARMA
International Classes:
C12Q1/34; C12N9/96; (IPC1-7): C12N9/96; C12Q1/34
Domestic Patent References:
JPS5523998A1980-02-20
JPS58162294A1983-09-26
JPS5985290A1984-05-17
JPS52128288A1977-10-27
Attorney, Agent or Firm:
Kazuyoshi Tsujimoto



 
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