Title:
環式置換-非対称シアニン染料
Document Type and Number:
Japanese Patent JP4684016
Kind Code:
B2
Abstract:
The invention relates to a method of analyzing the viability of a sample of cells using an aqueous solution comprising two fluorescent dyes. Dye I has the formula: where R2 is C1-6 alkyl; Z- is a biologically compatible counterion; X is O; S; Se; or NR15, where R15 is H or C1-6 alkyl; or CR16R17, where R16 and R17, which may be the same or different, are independently H or C1-6 alkyl, or the carbons of R16 and R17 taken in combination complete a five or six membered saturated ring; and the benzazolium is optionally further substituted; n=0, 1, or 2; Y is -CR3=CR4-; p and m=0 or 1, such that p+m=1; R5 is a C1-6 alkyl, C1-6 alkenyl, C1-6 polyalkenyl, C1-6 alkynyl or C1-6 polyalkynyl group; or R5 is an OMEGA; R3, R4, R6 and R7, which may be the same or different, are independently H; or a C1-6 alkyl, C1-6 alkenyl, C1-6 polyalkenyl, C1-6 alkynyl or C1-6 polyalkynyl group; or halogen; or -OR8, -SR8, -(NR8R9), where R8 and R9, which may be the same or different, are independently H; or alkyl groups having 1-6 carbons; or 1-2 substituted or unsubstituted alicyclic, heteroalicyclic, aromatic, or heteroaromatic rings, containing 1-4 heteroatoms, wherein the heteroatoms are O, N, or S; or R8 and R9 taken in combination are -(CH2)2-L-(CH2)2- where L=-O-, -NR10-, -CH2- or a single bond where R10 is H or an alkyl group having 1-6 carbons; or -OSO2R19 where R19 is C1-6 alkyl, or C1-6 perfluoroalkyl, or aryl; or an OMEGA; or R6 and R7, taken in combination are -(CH2)v- where v=3 or 4, or R6 and R7 form a fused aromatic ring that is optionally further substituted; such that at least one of R3, R4, R5, R6 and R7, or a substituent on the aromatic ring formed by R6 and R7, is an OMEGA; where OMEGA is a cyclic substituent that is attached by a single bond. Fluorescent Dye II selectively stains either viable or non-viable cells with a detectable fluorescent response that is different from the fluorescent response of Dye I. The stained cells are illuminated at a suitable absorption wavelength, and the fluorescent response is detected to distinguish viable and non-viable cells based on the fluorescent response.
Inventors:
Richard P. Hoagland
Kay Sam Wealth
Paul Jay Millard
Stephen Tea Yue
Bruce El Roth
Kay Sam Wealth
Paul Jay Millard
Stephen Tea Yue
Bruce El Roth
Application Number:
JP2005167584A
Publication Date:
May 18, 2011
Filing Date:
June 07, 2005
Export Citation:
Assignee:
Molecular Probes Inc
International Classes:
C07D215/22; C07D211/76; C09B23/00; C07D213/60; C07D213/61; C07D213/64; C07D215/16; C07D215/18; C07D215/227; C07D277/64; C07D413/06; C07D417/06; C07D417/14; C09B23/02; C09B23/04; C09B69/06; C12Q1/68; G01N21/64; G01N21/78; G01N27/26; G01N31/22; G01N33/48; G01N33/50; G01N33/52; G01N33/58
Foreign References:
| US4883867 | ||||
| US2646430 | ||||
| WO1993006482A1 |
Attorney, Agent or Firm:
Hatsushi Shimizu
Koichi Niimi
Koichi Niimi