Title:
ランダムフラグメント化により生成されたDNA分子を用いたDNA増幅および配列決定
Document Type and Number:
Japanese Patent JP2005535283
Kind Code:
A
Abstract:
The present invention is directed to methods to prepare a DNA molecule or a plurality of DNA molecules by random fragmentation. In some embodiments, the present invention regards preparing a template for DNA sequencing by random fragmentation. In specific embodiments, the random fragmentation comprises chemical fragmentation, mechanical fragmentation, or enzymatic fragmentation. In further specific embodiments, a universal sequence is attached to the 3′ end of the DNA fragments, such as by ligation of an adaptor sequence or by homopolymeric tailing with terminal deoxynucleotidyltransferase. In other embodiments, a library is prepared with methods of the present invention.
Inventors:
Makalob, Bradymia El.
Sleptova, Irina
Canberob, Emmanuel
Bruning, Eric
Sleptova, Irina
Canberob, Emmanuel
Bruning, Eric
Application Number:
JP2003551264A
Publication Date:
November 24, 2005
Filing Date:
November 13, 2002
Export Citation:
Assignee:
Rubycon Genomics, Inc.
International Classes:
C12N15/09; C07H19/00; C07H21/02; C07H21/04; C12P19/34; C12Q1/48; C12N; (IPC1-7): C12N15/09; C12Q1/48; C12Q1/68
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita