Title:
ＤＮＡメチル化編集用キットおよびＤＮＡメチル化編集方法
Document Type and Number:
Japanese Patent JP6500293
Kind Code:
B2
Abstract:
A DNA methylation editing kit comprises: (1) a fusion protein of inactivated CRISPR-associated endonuclease Cas9 (dCas9) having no nuclease activity and a tag peptide array in which plural tag peptides are linked by linkers, or an RNA or DNA coding therefor; (2) a fusion protein(s) of a tag peptide-binding portion and a methylase or demethylase, or an RNA(s) or DNA(s) coding therefor; and (3) a guide RNA(s) (gRNA(s)) comprising a sequence complementary to a DNA sequence within 1 kb of a desired site of methylation or demethylation, or a DNA(s) expressing the gRNA(s).
Inventors:
Hatada Deho
Morita Junyo
Takuro Horii
Morita Junyo
Takuro Horii
Application Number:
JP2017552723A
Publication Date:
April 17, 2019
Filing Date:
November 25, 2016
Export Citation:
Assignee:
Gunma University
International Classes:
C12N15/09; C12N15/63
Foreign References:
| WO2014152432A1 |
Other References:
TANENBAUM E. M. et al.,A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging,Cell,2014年,Vol.159,pp.635-646
MAEDER M. L. et al.,Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins,Nature Biotechnology,2013年,Vol.31, No.12,pp.1137-1142
MALI P. et al.,Cas9 as a versatile tool for engineering biology,Nature Methods,2013年,Vol.10, No.10,pp.957-963
山崎大賀 他,ゲノム編集技術を応用したペリセントロメアへの人為的・配列得的DNAメチル化誘導,BMB2015講演要旨集,2015年11月 6日,Vol.2015th,#1P0832
SHEN L. et al.,A single amino acid substitution confers enhanced methylation activity of mammalian Dnmt3b on chromatin DNA,Nucleic Acids Research,2010年,Vol.38, No.18,pp.6054-6064
CABANTOUS S. et al.,A new protein-protein interaction sensor based on tripartite split-GFP association,Scientific Reports,2013年,Vol.3,pp.2854/1-2854/9
SKELTON N. J. et al.,Origins of PDZ domain ligand specificity,The Journal of Biological Chemistry,2003年,Vol.278, No.9,pp.7645-7654
MAEDER M. L. et al.,Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins,Nature Biotechnology,2013年,Vol.31, No.12,pp.1137-1142
MALI P. et al.,Cas9 as a versatile tool for engineering biology,Nature Methods,2013年,Vol.10, No.10,pp.957-963
山崎大賀 他,ゲノム編集技術を応用したペリセントロメアへの人為的・配列得的DNAメチル化誘導,BMB2015講演要旨集,2015年11月 6日,Vol.2015th,#1P0832
SHEN L. et al.,A single amino acid substitution confers enhanced methylation activity of mammalian Dnmt3b on chromatin DNA,Nucleic Acids Research,2010年,Vol.38, No.18,pp.6054-6064
CABANTOUS S. et al.,A new protein-protein interaction sensor based on tripartite split-GFP association,Scientific Reports,2013年,Vol.3,pp.2854/1-2854/9
SKELTON N. J. et al.,Origins of PDZ domain ligand specificity,The Journal of Biological Chemistry,2003年,Vol.278, No.9,pp.7645-7654
Attorney, Agent or Firm:
Shinichi Sanuki
Yoshiyuki Kawaguchi
Yoshiyuki Kawaguchi