Title:
ＤＮＡ担持支持金ナノ粒子、その調製のためのプロセスおよびその使用
Document Type and Number:
Japanese Patent JP5896901
Kind Code:
B2
Abstract:
The present invention relates to DNA loaded gold nanoparticles embedded in sharp carbonaceous carriers useful for higher DNA delivery efficiently into plants. These nanogold embedded carbon matrices are prepared by heat treatment of biogenic intracellular gold nanoparticles. The DNA delivery efficiency is tested on model plants. These materials reveal good dispersion of the transport material, producing a greater number of GUS foci per unit area. The added advantages of the composite carrier are the lower plasmid and gold requirements. Plant cell damage with the prepared carbon supported particles is very minimal and can be gauged from the increased plant regeneration and transformation efficiency compared to that of the commercial micrometer sized gold particles. This can be attributed to the sharp edges that the carbon supports possess, which lead to better piercing capabilities with minimum damage.
Inventors:
Prasad Elbuy Bagatachula
Periyasami sham gum villain bear
Otara Tara Usharaji Abilash
Bashir Mohammad Khan
Periyasami sham gum villain bear
Otara Tara Usharaji Abilash
Bashir Mohammad Khan
Application Number:
JP2012523436A
Publication Date:
March 30, 2016
Filing Date:
August 03, 2010
Export Citation:
Assignee:
COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH
International Classes:
C12P3/00; B82Y5/00; B82Y40/00; C12M1/00; C12N15/00
Domestic Patent References:
| JP2009541593A | ||||
| JP2005168501A |
Foreign References:
| WO2011055888A1 |
Other References:
Torney F. et al.,nature nanotechnology,2007年,Vol.2,pp.295-300
Sivamani E. et al.,Plant Cell Rep,2008年11月18日,Vol.28,pp.213-221
Gericke M. et al.,hydrometallugy,2006年,Vol.83,pp.132-140
Zhang G. et al.,Biomaterials,2009年 1月 7日,Vol.30,pp.1928-1936
Lin X.M. et al.,Chem. Mater.,1999年,Vol.11,pp.198-202
Sivamani E. et al.,Plant Cell Rep,2008年11月18日,Vol.28,pp.213-221
Gericke M. et al.,hydrometallugy,2006年,Vol.83,pp.132-140
Zhang G. et al.,Biomaterials,2009年 1月 7日,Vol.30,pp.1928-1936
Lin X.M. et al.,Chem. Mater.,1999年,Vol.11,pp.198-202
Attorney, Agent or Firm:
Kiyoshi Mizuno
Hitoshi Kitamura
Tatsuo Itami
Hitoshi Kitamura
Tatsuo Itami