【発明の名称】シグナル伝達中間体のクローニング方法

Japanese Patent JP2002541779

A

The invention features a method of identifying a polypeptide which increases gene expression from a promoter, including (a) contacting a library of polypeptides with a cell that expresses a recombinant anti-cell death gene and that contains a reporter gene operably linked to a promoter (as shown in the figure), whereby expression of the reporter gene is increased if the library includes a polypeptide which increases gene expression from the promoter; (b) determining whether the reporter gene expression is increased in the cell as a result of contact with the polypeptide library; and (c) if reporter gene expression is increased, identifying a polypeptide of the library which increases reporter gene expression.

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Seed brian
Tin adrian

JP2000601196A

December 10, 2002

February 24, 2000

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The General Hospital Corporation

A61K38/00; A61P29/00; A61P31/12; A61P35/00; C07K14/47; C07K14/705; C12N5/10; C12N15/09; C12Q1/02; C12Q1/68; C12Q1/6897; G01N33/15; G01N33/50; G01N33/566; G01N33/68; (IPC1-7): C12N15/09; A61K38/00; A61P29/00; A61P31/12; A61P35/00; C07K14/47; C07K14/705; C12N5/10; C12Q1/02; C12Q1/68; G01N33/15; G01N33/50; G01N33/566; G01N33/68

Shimizu Hatsushi (1 person outside)

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