Title:
【発明の名称】検出可能な表現型のファージ形質導入による細菌の検出
Document Type and Number:
Japanese Patent JP2944694
Kind Code:
B2
Abstract:
Viable bacteria may be detected in biological samples by exposing bacterial cultures obtained from the samples to transducing particles having a known host range. Such transducing particles carry a heterologous gene capable of altering the phenotype of the bacteria in a readily detectable manner. For example, the transducing particles may carry an ice nucleation gene and the alteration of phenotype may be detected using an ice nucleation assay. By employing a panel of phage, unknown bacteria may be typed based on the pattern of reactivity observed. The method is particularly useful for detecting viable bacteria which may have been debilitated by exposure to sterilizing conditions, such as in food processing. The method is also useful for tracking a target bacteria in the ambient environment.
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Inventors:
UORUBAA HOORU KEI
GURIIN ROBAATO ERU
GURIIN ROBAATO ERU
Application Number:
JP51123789A
Publication Date:
September 06, 1999
Filing Date:
October 03, 1989
Export Citation:
Assignee:
DEII ENU EI PURANTO TEKUNOROJII CORP
International Classes:
C07K14/21; C12N1/21; C12N7/00; C12N9/24; C12N15/00; C12N15/09; C12Q1/02; C12Q1/04; G01N33/569; C12Q1/34; C12Q1/68; C12Q1/70; C12R1/38; C12R1/42; (IPC1-7): C12Q1/68; C12N1/21; C12N7/00; C12N15/09; C12Q1/04; C12Q1/70; G01N33/569
Domestic Patent References:
| JP6143998A | ||||
| JP59500747A |
Attorney, Agent or Firm:
Aoki Akira (3 outside)