Title:
【発明の名称】オリゴヌクレオチドの3 ́を標識するための方法、キット及び反応性支持体
Document Type and Number:
Japanese Patent JPH06503173
Kind Code:
A
Abstract:
In a first aspect, the invention involves a reactive support useful for automated synthesis of oligonucleotides. The reactive support comprises a label moiety (e.g. hapten) covalently bonded via a stable bond to a trifunctional spacer. The labeled trifunctional spacer complex is covalently bonded to a solid support via a cleavable bond. One arm of the trifunctional spacer attaches the solid phase; another arm attaches the label; while the third arm provides a hydroxyl group useful for synthesizing a labeled oligonucleotide. Upon synthesis, the cleavable bond is broken, yielding the labeled oligonucleotide. Methods for labeling oligonucleotides and useful kits are also described.
Inventors:
Huino, James Earl
Application Number:
JP50340692A
Publication Date:
April 07, 1994
Filing Date:
December 19, 1991
Export Citation:
Assignee:
Abbot Laboratories
International Classes:
C07H21/00; C12Q1/68; G01N33/532; C07H21/04; (IPC1-7): G01N33/532; C07H21/04
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)